In-stream product recovery of p-coumaric acid heterologously produced: Implementation of a continuous liquid-liquid extraction assisted by hollow fiber membrane contactor

This work aims to intensify trans-p-coumaric acid (p-CA) heterologous production. p-CA exhibits antimicrobial properties, low hydrosolubility, and retro-inhibition activity, making its production limited due accumulation in the broth. To overcome these limitations, an in-stream product recovery process (ISPR) is proposed consists a liquid-liquid extraction assisted by hollow fiber membrane contactor. pH, medium composition, solvent impacts on performances, were investigated prior implementation. The coupling of fermentation membrane-assisted was then studied. Although cells impaired shear stress with only 15% viable at end extractive fermentation, final concentration approximatively 89% control one, suggesting intensification if one factors proportion cells.